A serogrouping scheme for Campylobacter jejuni by slide agglutination method with formalin-treated antigens.
نویسندگان
چکیده
منابع مشابه
Serotyping scheme for Campylobacter jejuni and Campylobacter coli based on direct agglutination of heat-stable antigens.
Campylobacter is now the most frequently reported cause of gastrointestinal disease in England and Wales, yet few isolates are characterized beyond the genus level. The majority of isolates are Campylobacter jejuni (90%), with most of the remainder being Campylobacter coli. We describe an adaptation of the Penner serotyping scheme in which passive hemagglutination has been replaced by detection...
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Six different agglutinating antisera were obtained by immunizing rabbits with Formol-treated strains of Clostridium difficile. After appropriate absorption, these antisera were used to define six serogroups designated by the letters A, B, C, D, F, and G. Altogether, 315 strains of C. difficile from various origins were tested for slide agglutination by these antisera; 312 (99%) of them were agg...
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Results: Twenty one C. jejuni strains belonged to the following Penner’s serogroups: 8 strains were group A, 13 strains were group O. The remaining 5 strains were non-typable. RAPD-PCR analysis of 26 strains of Campylobacter jejuni yielded multiple amplification products in all of them. None of the strains processed by this method was non-typable. According to the number and sizes of amplificat...
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A simple, inexpensive slide agglutination test correctly identified the serogroup to which 38 Legionella pneumophilia strains belonged, and distinguished them from two atypical, Legionella-like organisms.
متن کاملComparison of the Denka Seiken slide agglutination method to the quellung test for serogrouping of Streptococcus pneumoniae isolates.
This study compared a slide agglutination test (Denka Seiken, Tokyo, Japan) to the "gold standard" quellung reaction (Pneumotest; Statens Serum Institut, Copenhagen, Denmark) for the serogrouping of pneumococci. Two hundred clinical isolates of Streptococcus pneumoniae were used for the comparison. Each assay was performed according to the manufacturer's instructions. There was an overall agree...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1987
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.42.499